Avicenna Journal of Neuro Psych Physiology Avicenna Journal of Neuro Psych Physiology Avicenna J Neuro Psych Physio http://www.avicennajnpp.com 2383-2436 2383-2444 10.5812/ajnpp. en jalali 2017 6 24 gregorian 2017 6 24 3 1
en 10.17795/ajnpp-34444 Morphological Effects of Hydroalcoholic Zingiber Officinalis Extract in the Murine Hippocampus of Male Rat Offspring Morphological Effects of Hydroalcoholic Zingiber Officinalis Extract in the Murine Hippocampus of Male Rat Offspring research-article research-article Background

The hippocampus is responsible for memory. A diet full of antioxidants improves brain damage and cognitive function. Regard the antioxidant effects of zingiber officinalis (ginger) and its flavonoids components.

Objectives

The aim of this study was to evaluate the effect of the extract of ginger on memory by using hippocampus tissue of the male offspring of rats.

Materials and Methods

In this study, 60 rats, 15 males and 45 females, were used. We separated pregnant female rats from males on the first day of pregnancy (determined by vaginal plug), and during days 16 - 18 of pregnancy, via intraperitoneal injection, three groups received hydroalcoholic extract of ginger, with low (200 mg/kg bw), medium (400 mg/kg bw), and high (800 mg/kg bw) concentration doses. The control group did not receive anything, and the sham group received normal saline during these days. Then at day 50, the males offspring in each group were sacrificed, their brains were removed, and the hippocampus sections were prepared for microscopic studies. Data was analyzed by SPSS 20 and by using one-way ANOVA and then a Tukey post-test (P < 0.05 considered as the significance level).

Results

This research showed that the number and thickness of pyramidal and granular layers of the CA1 and dentate gyrus areas of the hippocampus had increased in male offspring according to the increase in the ginger extract dose.

Conclusions

It seems as though ginger extract, which contains compounds such as gingerols, shogaols, and zingerone, can affect memory ability in rats through these compounds’ antioxidant properties by affecting embryonic acetylcholine content and place cells.

Background

The hippocampus is responsible for memory. A diet full of antioxidants improves brain damage and cognitive function. Regard the antioxidant effects of zingiber officinalis (ginger) and its flavonoids components.

Objectives

The aim of this study was to evaluate the effect of the extract of ginger on memory by using hippocampus tissue of the male offspring of rats.

Materials and Methods

In this study, 60 rats, 15 males and 45 females, were used. We separated pregnant female rats from males on the first day of pregnancy (determined by vaginal plug), and during days 16 - 18 of pregnancy, via intraperitoneal injection, three groups received hydroalcoholic extract of ginger, with low (200 mg/kg bw), medium (400 mg/kg bw), and high (800 mg/kg bw) concentration doses. The control group did not receive anything, and the sham group received normal saline during these days. Then at day 50, the males offspring in each group were sacrificed, their brains were removed, and the hippocampus sections were prepared for microscopic studies. Data was analyzed by SPSS 20 and by using one-way ANOVA and then a Tukey post-test (P < 0.05 considered as the significance level).

Results

This research showed that the number and thickness of pyramidal and granular layers of the CA1 and dentate gyrus areas of the hippocampus had increased in male offspring according to the increase in the ginger extract dose.

Conclusions

It seems as though ginger extract, which contains compounds such as gingerols, shogaols, and zingerone, can affect memory ability in rats through these compounds’ antioxidant properties by affecting embryonic acetylcholine content and place cells.

Ginger;Memory;Offspring;Male Rats;Hippocampus Ginger;Memory;Offspring;Male Rats;Hippocampus http://www.avicennajnpp.com/index.php?page=article&article_id=34444 Fariba Ghodrati Fariba Ghodrati Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran Minoo Mahmoodi Minoo Mahmoodi Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran; Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran. Tel: +98-9183138358 Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran; Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, IR Iran. Tel: +98-9183138358 Siamak Shahidi Siamak Shahidi Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Hamid Reza Ghadimipoure Hamid Reza Ghadimipoure Pathology Ward, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, IR Iran Pathology Ward, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, IR Iran